Relationship and you can regression data
We performed correlation analysis in each treatment modality group comparing the ISVs with the biodosimetric values measured at different time points. After Benjamini–Hochberg correction of multiple testing we found that 9 months after LDR therapy the chromosome aberration values (dicentrics + rings, chromatid deletions, total aberration and aberrant cell values) correlated positively and significantly with the largest ISVs, namely V1%, V1Gy. The correlations were weak, between 0.37 and 0.45 (Table 2). In HDR therapy, 3 months after radiotherapy dicentric plus rings correlated positively and weakly with most ISVs (Table 3). Aberrant cell number correlated with V150% at this time point as well (Spearman correlation coefficient 0.41). We found no correlations in any other time points https://datingranking.net/lds-singles-review/ of the follow-up. In the EBRT treatment group, the ISVs showed significant positive correlations with various chromosome aberrations directly after the radiotherapy, at the 3rd, 6th and at the 12th month. The aberrations which correlated with the most ISVs are shown in Table 4. Furthermore, the Spearman correlation coefficient for V10Gy are 0.52 and 0.51 for dicentrics + rings and aberrant cells at 3 months, respectively. The frequency of chromatid breaks and aberrant cells correlated with V10Gy (Spearman correlation coefficient 0.50 and 0.56) and aberrant cell number with V10% (0.49) at 6 months. Total aberrations (0.48) and aberrant cell number (0.43) showed correlation with V10Gy at 12 months.
Univariate regression analysis was performed to explain the variance of chromosome aberrations by variables of ISVs. Multiple regression cannot be used, because of the high collinearity of the variables. We sumini–Hochberg correction in Tables 4 and 5. In case of LDR therapy, we found, that none of the ISVs were significant predictors of chromosome aberrations (p > 0.2 R 2 < 6.4% at the ninth month after therapy). However, at the third month after HDR the predictive value (R 2 ) varied between 26.0 and 31.7, the best predictor being V100% (p = 0.004, R 2 = 31.7%) (Table 5). We also found significant regression models 12 months after the HDR irradiation, where we received no significant correlations. The R 2 ranged between 15.5% and 20.5% and the concerned aberrations were chromatid breaks, total aberrations and aberrant cell values (Table 5). After EBRT, the data of the regression models of time-points directly after radiotherapy and 9 months later are shown in Table 6. Furthermore, we also found significant regression models at 6 months after the therapy (V10% and chromatid deletions: R 2 = 19.5) and 12 months after the therapy (dicentrics and rings with V1% and V1Gy, R 2 = 20.1 and 18.7, respectively). On the other hand, less regression relation was found than with rank correlation. R 2 was between 17.3 and 49.4%, the highest value was calculated for the relationship of V10% and the total aberrations 9 months after the therapy.
Discussion
Brachytherapy, especially LDR treatment therapy is very hard to design from inside the cellphone countries once the amount beginning regarding LDR treatment continues as much as 12 months. Furthermore, various ranges regarding the numerous supply while the aftereffect of brand new flow are difficult to reproduce for the when you look at the vitro criteria. Whilst the routine knowledge of the new regulations out of radiobiology is even known within circumstances, the fresh new summarised impact of your numerous different aspects-opportunity, amount rates, medication date, irradiated regularity-into chromosome aberrations was unknown. In our investigation, i opposed the brand new biological effect of the different radiation therapy modalities regarding prostate cancer tumors treatment towards the mobile level, playing with lymphocytes originate from the brand new circulation. With this particular model, hence, we could prohibit this new bias aftereffect of the different irradiated quantities.
